![]() ![]() The SNAP-tag is a modified version of the human DNA repair enzyme alkylguanine-DNA alkyltransferase (AGT), which reacts specifically with O(6)-benzylguanine (BG)-modified molecules via irreversible transfer of an alkyl group to a cysteine residue. Self-labeling proteins such as the SNAP-tag are an innovative solution for addressing these challenges. However, most of these methods are relatively complicated and expensive and require several reaction steps. A variety of chemical, genetic and enzymatic site-specific conjugation methods have been developed for equipping antibodies with effector molecules to generate homogeneous antibody conjugates with tailored properties. Sticky ends from different BbsI sites may not be compatible.Abstract : Antibody-based diagnostic and therapeutic agents play a substantial role in medicine, especially in cancer management. Sticky ends from different BglI sites may not be compatible.Įfficient cleavage requires at least two copies of the SgrAI recognition sequence. G C C N N N N N G G C C G G N N N N N C C G PshAI quickly loses activity at 37☌, but can be used at 25☌ for long incubations. Sticky ends from different PasI sites may not be compatible. This recognition sequence is asymmetric, so ligating blunt ends generated by BmgBI will not always regenerate a BmgBI site. Prolonged incubation with BseRI may lead to degradation of the DNA.
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